AUTOIMMUNE HUMAN SPERM ANTIBODIES AND AGE IN MALES
نویسندگان
چکیده
منابع مشابه
Monoclonal antibodies to human sperm antigens.
To elucidate the molecular nature of human sperm autoantigens, attempts were made to raise monoclonal antibodies against these antigens, by hybridoma techniques. After successive immunizations with the particulate fractions of human sperm extract in BALB/c mice, the spleen cells were fused with P3-X63-Ag8 myeloma cells. Several clones and their subclones were obtained and shown by microplate ra...
متن کاملMonoclonal antibodies to human sperm antigens--II.
As part of our continuous effort to elucidate the biochemical and immunological nature of human sperm surface antigens, monoclonal antibodies to human spermatozoa were generated by improved hybridoma techniques. Following immunizations with the membrane fraction of human spermatozoa and cell fusions, hybrid cells were cultured in a semi-solid HAT-selection medium to maximize the number of monoc...
متن کاملProduction and immunolocalization of monoclonal antibodies against human sperm
In order to help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm surface antigens. The IgG fraction of serum from a donor with a spontaneous high titer of IgG-positive antisperm reactivity (as determined by immunobead binding) was purified by ammonium sulfate precipitation. This IgG preparation w...
متن کاملSenescent males carry premutagenic lesions in sperm.
As organisms age, DNA of somatic cells deteriorates, but it is believed that germ cells are protected from DNA-damaging agents. In recent years, this vision has been challenged by studies on humans indicating that genomic instability in germ cells increases with age. However, nothing is known about germ line senescence in wild animals. Here, we examine DNA damage in sperm of a wild vertebrate, ...
متن کاملAutoimmune anti-androgen-receptor antibodies in human serum.
Circulating autoantibodies to human and rat androgen receptors are present at high titers in the blood sera of some patients with prostate diseases. The antibodies from some serum samples were associated with a purified IgG fraction and interacted with the 3.8S cytosolic androgen-receptor complexes of rat ventral prostate to form 9- to 12S units. Other serum samples, however, formed 14- to 19S ...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1975
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0430145